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III degree; ??Uncontrolled hypotension and shock; ??Severe arrhythmia. _ Diagnosis and clinical classification of COVID -19 > Drugs can be prescribed by decision of the medical commission.
Treatment approaches are focused on symptomatic therapy, respiratory support.
• in pregnant women, in the postpartum period - the absence of obstetric complications.
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Psychological Intervention in COVID -19 Patients 2. A Trial of Lopinavir–Ritonavir in Adults Hospitalized with Severe Covid-19.
Virus cultivation must be carried out in a Biosafety Level 3 ( BSL - 3) laboratory.
Briefly, the procedure can be described as follows: Fresh sputum, stool, and other specimens are taken from the patient, after which they are inoculated into Vero - E 6 cells to culture the virus. The cytopathic effect (CE) is observed after 96 hours.
Detection of viral nucleic acid in the culture medium indicates successful cultivation. Determination of virus titer: After a 10-fold dilution of the viral inoculum, the TCID 50 is determined by the microcytopathic method.
Otherwise, the viability of the virus is determined by the plaque forming unit (PFU). > Pulse oximetry with SpO 2 measurement : a screening method for detecting respiratory failure, assessing the severity of hypoxemia in patients requiring respiratory support, and assessing the effectiveness of therapy. Nucleic acid testing is the preferred method for diagnosing SARS - CoV -2 infection . The test procedure according to the instructions in the kit is as follows: Samples are pretreated and the virus is lysed to isolate nucleic acids. Three specific SARS - CoV -2 genes , namely open reading frame 1 a / b ( ORF 1 a / b ), nucleocapsid protein ( N ) and envelope protein ( E ) genes, are then amplified by a real-time quantitative PCR technique . Amplified genes are detected by fluorescence intensity.
Let us name the criteria for positive results of the analysis for nucleic acids: A positive test for the ORF 1 a / b gene and (or) Buy Urispas (Flavoxate Hcl) 200mg online - General the health N gene ( E gene ).
(2) The staff should be divided into different working groups.